New Phytologist

We are now in the phenomic era. New, rapid screening approaches that provide quality data on plant physiological performance are a priority. A recently published advance, enabled by a next generation gas exchange system (LI‐6800, Li‐Cor, Lincoln, NE, USA), is the Rapid A/C_iResponse (RACiR: Stinziano et al., 2017). This technique aims to allow rapid (c. 12 min) determination of the A/c_i response (net CO₂ assimilation (A) response to intercellular CO₂concentration (c_i)), which previously required c. 40 min (standard A/c_i approach). Evaluating RACiR as a means for higher throughput phenotyping reveals offsets to commonly estimated parameters from A/c_i analyses; however, best practices for RACiR application and reporting should enable reliable comparisons with standard A/c_i measurements.